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Bacterial growth curve\Kinetic Curve. In autecological studies, the growth of bacteria (or other microorganisms, as protozoa, microalgae or yeasts) in batch culture can be modeled with four different phases: lag phase (A), log phase or exponential phase (B), stationary phase (C), and death phase (D).
Diauxic growth, meaning double growth, is caused by the presence of two sugars on a culture growth media, one of which is easier for the target bacterium to metabolize. The preferred sugar is consumed first, which leads to rapid growth, followed by a lag phase. [1] During the lag phase the cellular machinery used to metabolize the second sugar ...
[120] [121] The second phase of growth is the logarithmic phase, also known as the exponential phase. The log phase is marked by rapid exponential growth. The rate at which cells grow during this phase is known as the growth rate (k), and the time it takes the cells to double is known as the generation time (g). During log phase, nutrients are ...
The lag phase is not well known in microbiology, but it is speculated that this phase consists of the microorganism adjusting to its environment by synthesizing proteins specific for the surrounding habitat. [6] The log phase is the period where a culture experiences logarithmic growth until nutrients become scarce. The stationary phase is when ...
The Monod equation is a mathematical model for the growth of microorganisms. It is named for Jacques Monod (1910–1976, a French biochemist, Nobel Prize in Physiology or Medicine in 1965), who proposed using an equation of this form to relate microbial growth rates in an aqueous environment to the concentration of a limiting nutrient. [1][2][3 ...
The function also adheres to the sigmoid function, which is the most widely accepted convention of generally detailing a population's growth. Moreover, the function makes use of initial growth rate, which is commonly seen in populations of bacterial and cancer cells, which undergo the log phase and grow rapidly in numbers. Despite its ...
For protein expression and purification in bacteria it is recommended that protein induction and cell harvesting should be done at specific OD 600 (usually at the end of the log phase, OD 600 = 0.4). OD 600 is preferable to UV spectroscopy when measuring the growth over time of a cell population because at this wavelength, the cells will not be ...
Logarithmically growing bacteria differ from stationary phase bacteria with respect to the number of genome copies present in the cell, and this has implications for the capability to carry out an important DNA repair process. During logarithmic growth, two or more copies of any particular region of the chromosome may be present in a bacterial ...