Search results
Results from the WOW.Com Content Network
Bacterial growth curve\Kinetic Curve. In autecological studies, the growth of bacteria (or other microorganisms, as protozoa, microalgae or yeasts) in batch culture can be modeled with four different phases: lag phase (A), log phase or exponential phase (B), stationary phase (C), and death phase (D).
The Monod equation is a mathematical model for the growth of microorganisms. It is named for Jacques Monod (1910–1976, a French biochemist, Nobel Prize in Physiology or Medicine in 1965), who proposed using an equation of this form to relate microbial growth rates in an aqueous environment to the concentration of a limiting nutrient. [1][2][3 ...
The linear–log type of a semi-log graph, defined by a logarithmic scale on the x axis, and a linear scale on the y axis. Plotted lines are: y = 10 x (red), y = x (green), y = log (x) (blue). In science and engineering, a semi-log plot / graph or semi-logarithmic plot / graph has one axis on a logarithmic scale, the other on a linear scale.
Moreover, the function makes use of initial growth rate, which is commonly seen in populations of bacterial and cancer cells, which undergo the log phase and grow rapidly in numbers. Despite its popularity, the function initial rate of tumor growth is difficult to predetermine given the varying microcosms present with a patient, or varying ...
During this bacterial growth phase, the number of new cells appearing is proportional to the population. This terminological confusion between logarithmic growth and exponential growth may be explained by the fact that exponential growth curves may be straightened by plotting them using a logarithmic scale for the growth axis.
For protein expression and purification in bacteria it is recommended that protein induction and cell harvesting should be done at specific OD 600 (usually at the end of the log phase, OD 600 = 0.4). OD 600 is preferable to UV spectroscopy when measuring the growth over time of a cell population because at this wavelength, the cells will not be ...
Figure 1: A bi-phasic bacterial growth curve.. A growth curve is an empirical model of the evolution of a quantity over time. Growth curves are widely used in biology for quantities such as population size or biomass (in population ecology and demography, for population growth analysis), individual body height or biomass (in physiology, for growth analysis of individuals).
Subculturing can also be used for growth curve calculations (ex. generation time) [2] and obtaining log-phase microorganisms for experiments (ex. Bacterial transformation). [3] Typically, subculture is from a culture of a certain volume into fresh growth medium of equal volume, this allows long-term maintenance of the cell line.