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  2. Thin-layer chromatography - Wikipedia

    en.wikipedia.org/wiki/Thin-layer_chromatography

    Thin-layer chromatography. Thin-layer chromatography ( TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2] This is called the stationary phase. [2] The sample is deposited on the plate, which ...

  3. High-performance thin-layer chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_thin...

    Picture of HPTLC plate to identify different substances within a sample. High-performance thin-layer chromatography ( HPTLC) serves as an extension of thin-layer chromatography (TLC), offering robustness, simplicity, speed, and efficiency in the quantitative analysis of compounds. [1] This TLC-based analytical technique enhances compound ...

  4. Two-dimensional chromatography - Wikipedia

    en.wikipedia.org/wiki/Two-dimensional_chromatography

    Two-dimensional chromatography. Two-dimensional chromatography is a type of chromatographic technique in which the injected sample is separated by passing through two different separation stages. Two different chromatographic columns are connected in sequence, and the effluent from the first system is transferred onto the second column. [1]

  5. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Thin-layer chromatography is used to separate components of a plant extract, illustrating the experiment with plant pigments which gave chromatography its name. In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components.

  6. Hydrophobicity scales - Wikipedia

    en.wikipedia.org/wiki/Hydrophobicity_scales

    Pliska and his coworkers used thin layer chromatography to relate mobility values of free amino acids to their hydrophobicities. About a decade ago, another hydrophilicity scale was published, this scale used normal phase liquid chromatography and showed the retention of 121 peptides on an amide-80 column. [27]

  7. History of chromatography - Wikipedia

    en.wikipedia.org/wiki/History_of_chromatography

    The work of Martin and Synge also set the stage for high performance liquid chromatography, suggesting that small sorbent particles and pressure could produce fast liquid chromatography techniques. This became widely practical by the late 1960s (and the method was used to separate amino acids as early as 1960). Thin layer chromatography

  8. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    The partition coefficient principle has been applied in paper chromatography, thin layer chromatography, gas phase and liquid–liquid separation applications. The 1952 Nobel Prize in chemistry was earned by Archer John Porter Martin and Richard Laurence Millington Synge for their development of the technique, which was used for their ...

  9. Ion chromatography - Wikipedia

    en.wikipedia.org/wiki/Ion_chromatography

    Ion chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity to the ion exchanger. [1] It works on almost any kind of charged molecule —including small inorganic anions, [2] large proteins, [3] small nucleotides, [4] and amino acids.

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